J Exp Ther Oncol. Andrographolide, a potential cancer therapeutic agent isolated from Andrographis paniculata. Author information: 1 Discovery Research, Dr. Andrographolide, the major constituent of the extract is implicated towards its pharmacological activity. We studied the cellular processes and targets modulated by andrographolide treatment in human cancer and immune cells.
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In the body normal cells complete their life cycle by following an orderly path of growth, division and death. The process of programmed cell death is called apoptosis. When apoptosis breaks down, it leads to cancer. Chemotherapy is a major clinical treatment used for the control of advanced stages of malignancies and metastasis. But many cancer patients seek alternative and complementary methods of treatment because of the serious side effects of chemotherapy and radiation therapy.
Several herbs and plants with diversified pharmacological properties have been shown to have potential to prevent human cancers as they possess antitumor substances which have the potential to cure cancer without causing toxicity.
Andrographis paniculata is an annual herb belongs to family Acanthaceae. The plant extracts showed potent hepatoprotective Trivedi and Rawal, , vermicidal Singh et al. Silybum marianum is an annual or biennial erect herb belongs to the family Compositae. Different parts of this plant showed potent hepatoprotective Fraschini et al. The present study was undertaken to evaluate the anticancer activity of two medicinal plant s Andrographis paniculata and Silybum marianum aerial part and their combinational effects against five human cancer cell lines i.
D and confirmed at Botany Department, Dr. Gour Central University, Sagar M. All chemicals used were of analytical grade. The filtrate was evaporated to dryness using a rotary evaporator yield was The extracts were then stored below ambient temperature for further studies. Phytochemical test: Phyto chemical analysis was performed to detect various compounds such as tannins, flavonoids, alkaloids, steroids etc.
Maintained cultures were passage weekly and the culture medium was changed twice a week. Experiment: The human tumor cytotoxicity was determined by the protocols established by NCI Monks et al. The colorimetric assay estimated cell number indirectly by staining total cellular protein with the dye SRB. Single-cell suspensions were prepared by the treatment of cells with 0.
The viable cells were counted using a Coulter counter and diluted with RPMI medium and final densities of x cells mL-1 were obtained. After 24 h, the cells were treated with the extracts.
The supernatant was then discarded and the plates were washed 5 times with deionised water and then dried. Bounded stain was solubilised with Tris buffer 10 mM and the optical densities were read on an automated spectrophotometric plate reader Molecular Devices, USA at a wavelength of nm, Optical density OD of SRB in each well is directly proportional to the cell number.
Percentage inhibition was calculated by comparing the OD of control well with that of test sample: RESULTS Phytochemical study showed the presence of flavonoids, tannins, carbohydrate, cardiac glycosides, terpenes and steroids in both the plant extracts. Apart from this, alkaloids were present only in the extract of Andrographis paniculata while, saponins were present only in Silybum marianum extract Table 1.
The preliminary examination was done by observing the confluency of the test as well as control samples which showed cell growth inhibition in the test samples Fig. Table 1: Phyto chemical analysis of hydroalcoholic extract of A. Andrographis paniculata extract was found most effective against Ovcar cell line Again, the combination of both the plants showed an intermediate result for most of the cell line but the combination was most effective against the liver cell line HepG2 which showed better activity These medicinal plant s possess good immunomodulatory and antioxidant properties, leading to anticancer activities.
The antioxidant phytochemicals protect the cells from oxidative damage. Thus, consuming a diet rich in antioxidant plant material can provide health-protective effects. These natural products are supposed to minimize DNA damage by reacting with free radical s and in this way they can prevent cancer.
Phytochemicals, such as flavonols and flavonoids were investigated to determine chemoprevention activity against cancer Conese and Blasi, Phenols, polyphenols, flavonoids and their derivatives are ubiquitous in plants and have been found associated with the inhibition of atherosclerosis and cancer Cirla and Mann, Recent studies have reported antitumor effects of the flavonoids, quercetin, genistein and baicalein obtained from plant extracts Shoeb et al.
Similarly, alkaloids like schischkinnin and montamine have been isolated from the seeds of Centaurea schischkinii and Centaurea montana which showed anticancer property Yang and Wang, Flavonoids isolated from Plantago species were able to strongly inhibit the proliferation of human cancer cell lines Galvez et al.
Similarly, A. Further studies are required to isolate the active constituents involved in the antioxidant and hepatoprotective activity of the plant. Hancke, R. Burgos and G. Wikman, Prevention of common colds with Andrographis paniculata dried extract: A pilot double blind study. Phytomedicine, 4: Cirla, A. Mann, Combrestatins: From natural products to drug discovery.
Can Andrographis Help to Beat Cancer?
Can Andrographis Help to Beat Cancer? The stems and leaves of the plant are used to extract the active phytochemicals. Different active constituents have been identified, including andrographolide, deoxyandrographolide and other diterpenes. It was detected that andrographolide had the action of anti-tumor in gastric cancer, breast cancer, lung cancer and liver cancer. Andrographis Anticancer Activity Andrographolide, a diterpenoid lactone isolated from an herbal plant Andrographis paniculata, is known to possess anticancer effect. Andrographolide suppresses proliferation and triggers apoptosis in many types of cancer cells.
Andrographolide, a potential cancer therapeutic agent isolated from Andrographis paniculata.
In the body normal cells complete their life cycle by following an orderly path of growth, division and death. The process of programmed cell death is called apoptosis. When apoptosis breaks down, it leads to cancer. Chemotherapy is a major clinical treatment used for the control of advanced stages of malignancies and metastasis.
Received Jul 11; Accepted Oct This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This article has been cited by other articles in PMC. Abstract Salinity causes the adverse effects in all physiological processes of plants. The present study aimed to investigate the potential of salt stress to enhance the accumulation of the anticancer phytochemicals in Andrographis paniculata accessions. For this purpose, day-old plants were grown in different salinity levels 0.